abstract：:We report here the complete genome sequence of Citrobacter amalonaticus Y19 isolated from an anaerobic digester. PacBio single-molecule real-time (SMRT) sequencing was employed, resulting in a single scaffold of 5.58Mb. The sequence of a mega plasmid of 291Kb size is also presented. ...

journal_title：Journal of biotechnology

authors： Ainala SK,Seol E,Park S

更新日期：2015-10-10 00:00:00

abstract：:Glycosylation is one of the most important tailoring reactions for natural products. It typically exerts profound direct or indirect effects on their biological activity. The dihydrochalcone phloretin and its known sugar derivatives, particularly phlori(d)zin, have been shown to influence various cellular processes. W...

journal_title：Journal of biotechnology

authors： Overwin H,Wray V,Hofer B

更新日期：2015-10-10 00:00:00

abstract：:Biosurfactants are surface-active agents produced by microorganisms and show increasing significance in various industrial applications. A great variety of these secondary metabolites are described to occur within actinomycetes, amongst trehalose lipids and oligosaccharide lipids produced by the family Tsukamurellacea...

journal_title：Journal of biotechnology

authors： Kügler JH,Kraft A,Heißler S,Muhle-Goll C,Luy B,Schwack W,Syldatk C,Hausmann R

更新日期：2015-10-10 00:00:00

abstract：:Bacillus velezensis G341 can suppress plant pathogens by producing antagonistic active compounds including bacillomycin D, fengycin, and (oxy) difficidin. The complete genome sequence of this bacterium was characterized by one circular chromosome of 4,009,746bp with 3953 open reading frames. The genome contained 36 ps...

journal_title：Journal of biotechnology

authors： Lee HH,Park J,Lim JY,Kim H,Choi GJ,Kim JC,Seo YS

更新日期：2015-10-10 00:00:00

abstract：:The solvent resistance capacity of Pseudomonas putida S12 was applied by using the organism as a host for biocatalysis and through cloning and expressing solvent resistant pump genes into Escherichia coli. P. putida S12 expressing toluene ortho mononooxygenase (TOM-Green) was used for 1-naphthol production in a water-...

journal_title：Journal of biotechnology

authors： Janardhan Garikipati SV,Peeples TL

更新日期：2015-09-20 00:00:00

abstract：:2'-Fucosyllactose (2-FL) is one of most abundant functional oligosaccharides in human milk, which is involved in many biological functions for human health. To date, most microbial systems for 2-FL production have been limited to use Escherichia coli JM strains since they cannot metabolize lactose. In this study, E. c...

journal_title：Journal of biotechnology

authors： Chin YW,Kim JY,Lee WH,Seo JH

更新日期：2015-09-20 00:00:00

abstract：:Bifidobacterium catenulatum JCM 1194(T) was isolated from human feces. This paper is the first report demonstrating the fully sequenced and completely annotated genome of a B. catenulatum strain. ...

journal_title：Journal of biotechnology

authors： Morita H,Toh H,Oshima K,Nakano A,Yamashita N,Iioka E,Arakawa K,Suda W,Honda K,Hattori M

更新日期：2015-09-20 00:00:00

abstract：:The objective of this work was to construct a non-pathogenic Klebsiella pneumonia strain that can produce optically high concentrated (R,R)-2,3-BDO. A K. pneumonia mutant lacking the pathogenic factor was used as the host strain. In order to construct a K. pneumonia strain that would biosynthesize high concentrated (R...

journal_title：Journal of biotechnology

authors： Lee S,Kim B,Yang J,Jeong D,Park S,Lee J

更新日期：2015-09-10 00:00:00

abstract：:Lactulose and galacto-oligosaccharides (GOS) are well recognized prebiotics derived from lactose. In the synthesis of lactulose with β-galactosidases GOS are also produced, but the ratio of lactulose and GOS in the product can be tuned at will, depending on the operation conditions, so to obtain an optimal product dis...

journal_title：Journal of biotechnology

authors： Guerrero C,Vera C,Acevedo F,Illanes A

更新日期：2015-09-10 00:00:00

abstract：:Linoleate 13-hydratase from Lactobacillus acidophilus LMG 11470 converted linoleic acid to hydroxyl fatty acid, which was identified as 13S-hydroxy-9(Z)-octadecenoic acid (13-HOD) by GC-MS and NMR. The expression of linoleate 13-hydratase gene in Escherichia coli was maximized by using pACYC plasmid and super optimal ...

journal_title：Journal of biotechnology

authors： Park JY,Lee SH,Kim KR,Park JB,Oh DK

更新日期：2015-08-20 00:00:00

abstract：:Paenibacillus riograndensis is a Gram-positive rhizobacterium which exhibits plant growth promoting activities. It was isolated from the rhizosphere of wheat grown in the state of Rio Grande do Sul, Brazil. Here we announce the complete genome sequence of P. riograndensis strain SBR5(T). The genome of P. riograndensis...

journal_title：Journal of biotechnology

authors： Brito LF,Bach E,Kalinowski J,Rückert C,Wibberg D,Passaglia LM,Wendisch VF

更新日期：2015-08-10 00:00:00

abstract：:Physiologically relevant in vitro models can serve as biological analytical platforms for testing novel treatments and drug delivery systems. We describe the first steps in the development of a 3D human brain tumour co-culture model that includes the interplay between normal and tumour tissue along with nutrient gradi...

journal_title：Journal of biotechnology

authors： Ivanov DP,Parker TL,Walker DA,Alexander C,Ashford MB,Gellert PR,Garnett MC

更新日期：2015-07-10 00:00:00

abstract：:Aptamers are nucleic acid-based high affinity ligands that are able to capture their corresponding target through molecular recognition. In this study, several DNA aptamers with high affinity and specificity for β-glucuronidases (PGUS-E) were obtained by our modified SELEX method. Among them, Apt5 and Apt9 were select...

journal_title：Journal of biotechnology

authors： Qiao L,Lv B,Feng X,Li C

更新日期：2015-06-10 00:00:00

abstract：:The keto ester reductase from Candida glabrata, designated as CgKR1, is a highly versatile biocatalyst with a broad substrate spectrum. Its substrate preference was altered by rational design of the active pocket for bioreduction of aromatic α-keto esters in our previous work. However, its practical application is sti...

journal_title：Journal of biotechnology

authors： Huang L,Xu JH,Yu HL

更新日期：2015-06-10 00:00:00

abstract：:Following its introduction in 1967, rifampicin has become a mainstay of therapy in the treatment of tuberculosis, leprosy and many other widespread diseases. Its potent antibacterial activity is due to specific inhibition of bacterial RNA polymerase. However, resistance to rifampicin was reported shortly after its int...

journal_title：Journal of biotechnology

authors： Alifano P,Palumbo C,Pasanisi D,Talà A

更新日期：2015-05-20 00:00:00

abstract：:Group I grass pollen allergens are major contributors to grass pollen-related seasonal allergic rhinitis, and as such a primary target for allergen specific immunotherapy. In this study the potential therapeutic role of oral application of Lactobacillus plantarum WCFS1, directing cell wall attachment of the recombinan...

journal_title：Journal of biotechnology

authors： Minic R,Gavrovic-Jankulovic M,Petrusic V,Zivkovic I,Eijsink VG,Dimitrijevic L,Mathiesen G

更新日期：2015-04-10 00:00:00

abstract：:Streptomyces lividans TK24 is the standard host for the heterologous expression of a number of different proteins and antibiotic-synthesizing enzymes. As such, it is often used as an experimental microbial cell factory for the production of secreted heterologous proteins including human cytokines and industrial enzyme...

journal_title：Journal of biotechnology

authors： Rückert C,Albersmeier A,Busche T,Jaenicke S,Winkler A,Friðjónsson ÓH,Hreggviðsson GÓ,Lambert C,Badcock D,Bernaerts K,Anne J,Economou A,Kalinowski J

更新日期：2015-04-10 00:00:00

abstract：:Semi-synthetic β-lactam antibiotics (SSBAs) are one of the most important antibiotic families in the world market. Their enzymatic synthesis can be catalyzed by penicillin G acylases (PGAs). In this study, to improve enzymatic synthesis of ampicillin, site-saturating mutagenesis was performed on three conserved amino ...

journal_title：Journal of biotechnology

authors： Deng S,Su E,Ma X,Yang S,Wei D

更新日期：2015-04-10 00:00:00

abstract：:Threonine aldolase (TA) catalyzes a reversible reaction, in which threonine is decomposed into glycine and acetaldehyde. The same enzyme can be used to catalyze aldol reaction between glycine and a variety of aromatic and aliphatic aldehydes, thus creating various alpha-amino-alcohols. Therefore, TA is a very promisin...

journal_title：Journal of biotechnology

authors： Wieteska L,Ionov M,Szemraj J,Feller C,Kolinski A,Gront D

更新日期：2015-04-10 00:00:00

abstract：:Potato tuber dormancy release is a critical development process that allows potato to produce new plant. The first Illumina RNA sequencing to generate the expressed mRNAs at dormancy tuber (DT), dormancy release tuber (DRT) and sprouting tuber (ST) was performed. We identified 26,639 genes including 5,912 (3,450 up-re...

journal_title：Journal of biotechnology

authors： Liu B,Zhang N,Wen Y,Jin X,Yang J,Si H,Wang D

更新日期：2015-03-20 00:00:00

abstract：:Native enzyme and a mutant containing an extra disulphide bridge of recombinant Saccharomycopsis fibuligera R64 α-amylase, designated as Sfamy01 and Sfamy02, respectively, have successfully been overexpressed in the yeast Pichia pastoris KM71H. The purified α-amylase variants demonstrated starch hydrolysis resulting i...

journal_title：Journal of biotechnology

authors： Natalia D,Vidilaseris K,Ismaya WT,Puspasari F,Prawira I,Hasan K,Fibriansah G,Permentier HP,Nurachman Z,Subroto T,Dijkstra BW,Soemitro S

更新日期：2015-02-10 00:00:00

abstract：:The aim of this study was to improve the ability of Aspergillus terreus lipase to separate the racemic ketoprofen vinyl ester into individual enantiomers using hollow self-assembly alginate-graft-poly(ethylene glycol)/α-cyclodextrins (Alg-g-PEG/α-CD) spheres as enzyme immobilization carriers. The morphology and size o...

journal_title：Journal of biotechnology

authors： Hu C,Wang N,Zhang W,Zhang S,Meng Y,Yu X

更新日期：2015-01-20 00:00:00

abstract：:Recombinant enhanced green fluorescent protein (eGFP) is used as a marker in numerous applications in biomedical research and diagnostics. For these applications, the macromolecule needs to be provided in a highly purified form. The conventional purification process of eGFP usually consists of multiple subsequent prep...

journal_title：Journal of biotechnology

authors： Hekmat D,Maslak D,Freiherr von Roman M,Breitschwerdt P,Ströhle C,Vogt A,Berensmeier S,Weuster-Botz D

更新日期：2015-01-20 00:00:00

abstract：:The haloarchaeon Haloferax mediterranei is able to grow in the presence of different inorganic and organic nitrogen sources by means of the assimilatory pathway under aerobic conditions. In order to identify genes of potential importance in nitrogen metabolism and its regulation in the halophilic microorganism, we hav...

journal_title：Journal of biotechnology

authors： Esclapez J,Pire C,Camacho M,Bautista V,Martínez-Espinosa RM,Zafrilla B,Vegara A,Alcaraz LA,Bonete MJ

更新日期：2015-01-10 00:00:00

abstract：:Enterobacter cloacae is a facultative anaerobic bacterium to be an important cause of nosocomial infection. However, the isolated E. cloacae GGT036 showed higher furfural-tolerant cellular growth, compared to industrial relevant strains such as Escherichia coli and Corynebacterium glutamicum. Here, we report the compl...

journal_title：Journal of biotechnology

authors： Gong G,Um Y,Park TH,Woo HM

更新日期：2015-01-10 00:00:00

abstract：:The Gram-positive Corynebacterium glutamicum is auxotrophic for biotin. Besides the biotin uptake system BioYMN and the transcriptional regulator BioQ, this bacterium possesses functional enzymes for the last three reactions of biotin synthesis starting from pimeloyl-CoA. Heterologous expression of bioF from the Gram-...

journal_title：Journal of biotechnology

authors： Peters-Wendisch P,Götker S,Heider SA,Komati Reddy G,Nguyen AQ,Stansen KC,Wendisch VF

更新日期：2014-12-20 00:00:00

abstract：:Biomass-derived d-xylose represents an economically interesting substrate for the sustainable microbial production of value-added compounds. The industrially important platform organism Corynebacterium glutamicum has already been engineered to grow on this pentose as sole carbon and energy source. However, all current...

journal_title：Journal of biotechnology

authors： Radek A,Krumbach K,Gätgens J,Wendisch VF,Wiechert W,Bott M,Noack S,Marienhagen J

更新日期：2014-12-20 00:00:00

abstract：:The neotype strain Methanobacterium formicicum MF(T) (DSM1535), a hydrogenotrophic methanogenic Archaeon, was isolated from a domestic sewage sludge digestor in Urbana (IL, USA). Here, the complete genome sequence of the methanogen is reported. The genome is 2,478,074bp in size, featuring a GC content of 41.23%. M. fo...

journal_title：Journal of biotechnology

authors： Maus I,Stantscheff R,Wibberg D,Stolze Y,Winkler A,Pühler A,König H,Schlüter A

更新日期：2014-12-20 00:00:00

abstract：:Reteplase is the third generation of thrombolytic medicine and has many advantages over commercial t-PA. However, over-expressing recombinant reteplase in E. coli always accumulates as inclusion bodies due to nine pairs of disulfide bonds formation that is the main obstacle for correct folding. In this paper, in order...

journal_title：Journal of biotechnology

authors： Zhuo XF,Zhang YY,Guan YX,Yao SJ

更新日期：2014-12-20 00:00:00

abstract：:A PHA producing microbial culture dominated by Plasticicumulans acidivorans was enriched in a pilot plant using fermented wastewater from the Mars candy bar factory. The pilot plant comprised (1) anaerobic fermentation, (2) enrichment of a PHA-producing microbial community and (3) accumulation for maximization of the ...

journal_title：Journal of biotechnology

authors： Tamisa J,Lužkov K,Jiang Y,van Loosdrecht MC,Kleerebezem R

更新日期：2014-12-20 00:00:00

abstract：:In their natural habitats microorganisms live in multi-species communities, in which the community members exhibit complex metabolic interactions. In contrast, biotechnological production processes catalyzed by microorganisms are usually carried out with single strains in pure cultures. A number of production processe...

journal_title：Journal of biotechnology

authors： Jagmann N,Philipp B

更新日期：2014-12-20 00:00:00

abstract：:Yarrowia lipolytica can accumulate large amounts of storage lipids and has considerable potential for the production of polyunsaturated fatty acids and other lipids for biofuels. When the nitrogen source is exhausted in the medium, the key intermediate, citrate, is converted to acetyl-CoA by ATP:citrate lyase (ACL) fo...

journal_title：Journal of biotechnology

authors： Zhang H,Zhang L,Chen H,Chen YQ,Chen W,Song Y,Ratledge C

更新日期：2014-12-20 00:00:00

abstract：:In this work, a proof of concept elastin-like polypeptide-Z domain fusion (ELP-Z) based monoclonal antibody (mAb) affinity precipitation process is developed using scaled-down filtration techniques. Tangential flow filtration (TFF) is examined for the recovery of ELP-Z-mAb precipitates formed during the mAb binding st...

journal_title：Journal of biotechnology

authors： Sheth RD,Bhut BV,Jin M,Li Z,Chen W,Cramer SM

更新日期：2014-12-20 00:00:00

abstract：:Combining the isochorismate synthase EntC and the chorismatase FkbO in a sequential enzyme cascade provides a useful system for the biocatalytic production and subsequent purification of isochorismate from an isochorismate/chorismate mixture. FkbO has a strict preference for chorismate - isochorismate is not accepted ...

journal_title：Journal of biotechnology

authors： Hubrich F,Müller M,Andexer JN

更新日期：2014-12-10 00:00:00

abstract：:The growth of bacteria as structured aggregates termed biofilms leads to their protection from harsh environmental conditions such as physical and chemical stresses, shearing forces, and limited nutrient availability. Because of this highly adapted ability to survive adverse environmental conditions, bacterial biofilm...

journal_title：Journal of biotechnology

authors： Taylor PK,Yeung AT,Hancock RE

更新日期：2014-12-10 00:00:00

abstract：:The sesquiterpene (+)-valencene is an aroma compound of citrus fruits and is used to flavor foods and drinks. Biosynthesis of (+)-valencene starts from farnesyl pyrophosphate, an intermediate of carotenoid biosynthesis. Corynebacterium glutamicum, the workhorse of the million-ton scale amino acid industry, is naturall...

journal_title：Journal of biotechnology

authors： Frohwitter J,Heider SA,Peters-Wendisch P,Beekwilder J,Wendisch VF

更新日期：2014-12-10 00:00:00

abstract：:We report the complete genome sequence of Amycolatopsis japonica MG417-CF17(T) (=DSM 44213(T)) which was identified as the producer of (S,S)-N,N'-ethylenediaminedisuccinic acid during a screening for phospholipase C inhibitors. The genome of A. japonica MG417-CF17(T) consists of two replicons: the chromosome (8,961,31...

journal_title：Journal of biotechnology

authors： Stegmann E,Albersmeier A,Spohn M,Gert H,Weber T,Wohlleben W,Kalinowski J,Rückert C

更新日期：2014-11-10 00:00:00

abstract：:Conventional approaches to differential gene expression comparisons assume equal cellular RNA content among experimental conditions. We demonstrate that this assumption should not be universally applied because total RNA yield from a set number of cells varies among experimental treatments of the same Chinese Hamster ...

journal_title：Journal of biotechnology

authors： Fomina-Yadlin D,Du Z,McGrew JT

更新日期：2014-11-10 00:00:00

abstract：:In this work, we fabricated a system of integrated self-assembled layer of organosilane 3-mercaptopropyltrimethoxy silane (MPTS) on the screen printed electrode (SPE) and electrochemically deposited gold nanoparticle for Salmonella typhi detection employing Vi gene as a molecular marker. Thiolated DNA probe was immobi...

journal_title：Journal of biotechnology

authors： Das R,Sharma MK,Rao VK,Bhattacharya BK,Garg I,Venkatesh V,Upadhyay S

更新日期：2014-10-20 00:00:00

abstract：:The genome of the biotechnologically important solventogenic Clostridium saccharobutylicum NCP 262 contains two operons coding for genes of presumed type I RM systems belonging to the families A and C. They represent a limiting factor for the development of transformation and conjugation protocols. We established an e...

journal_title：Journal of biotechnology

authors： Lesiak JM,Liebl W,Ehrenreich A

更新日期：2014-10-20 00:00:00